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Recipes from The Spin Doctor's Cook Book
Pre-Rinsing Procedure
Materials
NALGENE Centrifuge Filters
Buffer or distilled water
0.1 M NaOH (optional)
Method
  1. Centrifuge 0.4ml (Mini), 3ml (Midi) or 5 ml (Maxi) of either an appropriate buffer or distilled water until half has passed through the membrane.
  2. If further per rinsing is required, centrifuge 0.4ml (Mini), 3ml (Midi) or 5 ml (Maxi) of 0.1 M NaOH and repeat rinse procedure above.
  3. Do not allow the membrane to dry out before use. If the device is not for immediate use, place distilled water in the challenge reservoir to cover the membrane, and store the device at -4o C.
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