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Applications Guide for NALGENE Centrifuge Filters
Spin Filter Recipes
Purification of PCR Products
Desalting of Nucleic Acids by Discontinuous Diafiltration
Prerinsing Procedure
Ask The Spin Doctor
FAQ's
Spin Filter Application Table
The table below is meant as a guideline. In general, the MWCO should be well below the weight of the challenge solute to ensure high recovery. The best filter size(s) to use will be determined empirically.


APPLICATIONS 4k MWCO 10k MWCO 30k MWCO 100k MWCO 0.2
micron pore size
0.45
micron pore size
Protein Concentration    
Protein Purification    
Protein Isolation from Cell Lysates  
Protein Removal Prior to HPLC        
Desalting of Column Fractions    
Protein Concentration for Electrophoresis    
Removal of Labelled Amino Acids    
Peptide Concentration        
Antibody Concentration        
Virus Concentration        
Concentration/Desalting of Nucleic Acids    
Concentration of Oligonucleotides
Removal of Primers from Amplified DNA          
Removal of Linkers Prior to Cloning        
Removal of Labelled Nucleotides      
Protein Recovery from Polyacrylamide Gel
DNA Recovery from Agarose Gel
RNA Recovery form Polyacrylamide Gel
Antibody Purification from Hybridoma Cells      
Oligonucleotide from Polyacrylamide Gel
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