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| Desalting of Nucleic Acids by Discontinuous
Diafiltration.
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| Materials
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NALGENE
Mini-Centrifuge Filter of appropriate MWCO (see
application table).
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Distilled
water or appropriate buffer/diluent.
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Micropipette
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Microcentrifuge
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| Method
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Introduce up to 500ul of sample into the challenge reservoir of the NALGENE
Mini Centrifuge Filter.
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Centrifuge at up to 13,000g.
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Remove filtrate from tube.
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Add diluent to the retentate, to a volume not exceeding 500ul and centrifuge
again.
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This process may be repeated with the salt removal proportional to the dilution
factor.
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Remove insert containing sample concentrate from the tube once satisfactory
salt removal has been carried out. Invert insert and place in a new centrifuge
tube.
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Spin for 1000g x 3 min to recover nucleic acid.
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Remove insert and discard. Cap tube to store nucleic acid.
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