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Before labware contaminated with infectious or toxic materials is removed from
the work area, it should be sterilized appropriately. Autoclaving is the
preferred method for sterilization; however, any method of chemical
disinfecting or heat sterilization appropriate for the particular plastic may
be used (see Sterilizing Plastics). Liquid
waste containing biohazardous materials must always be decontaminated before
disposal.
Labware that is contaminated with both biohazardous and radioactive material
must first be sterilized. Methods for removing radioactive material depend on
the isotope used, its quantity, half-life, material and solubility. For routine
decontamination of non-infectious/non-toxic materials, first soak in
decontaminant/cleaner for 24 hours at room temperature. Follow with several
rinsings in distilled water. To accelerate decontamination, increase the
cleaner concentration and solution temperature. Agitation and careful scrubbing
with non-abrasive materials will also speed this process. Be particularly
careful not to scratch PC. Always dispose of radioactive wastes and effluents
properly.
For additional information on handling contaminated labware, contact your
Biosafety/Radiation Safety Office, or refer to NIH Biohazards Safety Guide,
Laboratory Safety Monograph, and Radiation Safety Guide.
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